Building high affinity

Assembly of high-affinity insulin receptor agonists and antagonists from peptide building blocks.

Insulin is thought to elicit its effects by crosslinking the two extracellular alpha-subunits of its receptor, thereby inducing a conformational change in the receptor, which activates the intracellular tyrosine kinase signaling cascade. Previously we identified a series of peptides binding to two discrete hotspots on the insulin receptor. Here we show that covalent linkage of such peptides int...

High-throughput affinity mass spectrometry.

Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolating target protein(s) from complex biological fluids for mass spectrometric analysis. The resulting high-content mass spectrometry (MS) data show the unique MS protein signatures (wild-type, posttranslationally modified, as well as genetically modified forms of the protein target) that are present within a biologica...

High-Affinity Interactions between Peptides

Preparation and Characterisation of Immunoadsorbents for High Performance Liquid Affinity Chromatography (HPLAC)

The efficacy of activation methods and coupling were studied in the context of performance in batch and fixed bed binding experiments utilizing cell culture fluids or blood plasma as feedstock. Conclusions were drawn regarding selection of solid phase according to pore size, rigidity, pH stability, Chemistry of derivation and activation, and gross concentration of immobilized ligand require...

High Precision Building Documentation:

In this paper a methodology of building documentation is presented which is focused on the development of a 3D GIS. The methodology is based on the description of buildings by means of hierarchically structured architectural elements. This form fulfills the requirements of spatial databases and thus enables the implementation in 3D information systems. INTRODUCTION A complete documentation of a...